Masayuki izumi biography sampler

Isopeptide bond formation mediated by δ-selenolysine for chemical ubiquitination

1 Introduction

Protein ubiquitination is a major post-translational modification in Eukaryotes and is involved in nearly all biological processes, i.e., protein degradation, DNA repair, and cell signaling. Hence, unraveling its precise role in health and disease is important (Chaugule and Walden, ; Swatek and Komander, ). Ubiquitin (Ub) is a mer protein found in almost all Eukaryotes. In protein ubiquitination, the C-terminal carboxylate of Ub is attached to the µ-amino group of the lysine residue in the acceptor protein through an isopeptide bond. Ub can form homopolymers, heteropolymers, and branched polymers through its seven Lys residues and one N-terminal Met. This structural diversity allows the formation of the Ub code (Komander and Rape, ; Swatek and Komander, ).

To gain precise insights into the complex Ub codes formed by diverse polyUb chains, researchers frequently employ chemical probes with polyUb chains having a defined linkage attached to a defined position of the acceptor protein (Mulder et al., ). Certain polyUb chains with defined linkage and length can be prepared using specific E2 or E3 enzymes. However, this method has some limitations, e.g., polyUb chains attach to the acceptor protein (Faggiano et al., ). Chemical protein synthesis has been extensively developed to overcome these limitations (Mali et al., ; Gui et al., ; Huppelschoten and van der Heden van Noort, ). Three strategies have been developed for the chemical synthesis of native isopeptide bonds: Auxiliary mediated ligation, first reported by Muir (Chatterjee et al., ); ´-mercaptolysine- (Kumar et al., ) or ³-mercaptolysine- (Yang et al., ; Merkx et al., ) mediated ligation; and isoUb ligation (Tang et al., ).

´-Mercaptolysine-mediated ligation has been extensively used for the synthesis of diUb chains (Kumar et al., ), tetra-ubiquitinated peptides (Bavikar et al., ),

  • 職務経験. Professor. 高知大学. 年4月 – 現在

    • 研究人才詳細資料

    出版年月著作類別著作名稱作者收錄出處
    研討會論文 AIT support equipment design for METISYu Chieh Huang, Remko Stuik, Dirk Lesman, Shiang-Yu Wang, Ping-Jie Huang, Jia-Ruei Nian, Hsin-Yo Chen, Ta-Shun Wei, Shu-Fu Hsu, Gilles Otten, Felix Bettonvil, Bernhard R. Brandl
    研討會論文 Final design and status of the Mid-IR ELT Imager and Spectrograph, METISBernhard R. Brandl, Felix Bettonvil, Roy van Boekel, Adrian Glauser, Sascha P. Quanz, Olivier Absil, Markus Feldt, Paulo J. V. Garcia, Alistair Glasse, Manuel Guedel, Lucas Labadie, Michael Meyer, Eric Pantin, Shiang-Yu Wang, Hans van Winckel
    研討會論文 Implementation plans for the data reduction pipeline for METIS at the ELTKieran Leschinski, Hugo Buddelmeijer, Oliver Czoske, Gilles Otten, Martin Balaz, Fabian Haberhauer, Jennifer Karr, Wolfgang Kausch, Thomas Marquart, Nadeen Sabha, Chi-Hung Yan, Norbert Przybilla, Shiang-Yu Wang, Werner Zeilinger
    研討會論文 Innovations in the design and construction of the GMT-Consortium Large Earth Finder (G-CLEF), a first-light instrument for the Giant Magellan Telescope (GMT)Andrew Szentgyorgyi, Sagi Ben-Ami, Jae Sok Oh, Chan Park, Daniel Baldwin, Jacob Bean, Alex Bichkovsky, Patricia Brennan, Daniel Catropa, Chueh-Yi Chou, Moo-Young

    Search code, repositories, users, issues, pull requests

    OHP is Hacker's Presentation writer with Markdown and CSS

    • () - Font size (default: )
    • - Default configuration for each pages
      • () - Default class(es)
    • - Default configuration for page numbers
      • () - Whether to render the page numbers (default: )
      • () - Class(es) of page number elements (default: )
      • () - Pgae number for the first page (default: )
    fontSize: 32page: className: pagepageNumber: enable: true# Title
    # Page 1<!-- newpage --># Page 2
    • () - Class(es) of the page element
    <!-- page className="title" --># Title<!-- newpage --><!-- page className="toc" --># Table of Contents
  • In this study, we introduce a

    • Search code, repositories, users, issues, pull requests

    • Bletia: Promisified BluetoothGatt wrapper library(including JDeferred)
    • RxBletia: RxJava binding APIs for Android's BluetoothGatt

    Use Bletia:

    dependencies { // use Bletia compile 'd:bletia' }

    Use RxBletia:

    dependencies { // use RxBletia compile 'd:bletia' compile 'vex:rxjava' }
    // Pass application context to bletia = newBletia(context);
    // BluetoothDevice t(device);
    // BluetoothGattCharacteristic characteristic Notification(characteristic, true) .then(newDoneCallback<BluetoothGattCharacteristic>() { @OverridepublicvoidonDone(BluetoothGattCharacteristicresult) { // Call when the request was successfully. } }) .fail(newFailCallback<BletiaException>() { @OVerridepublicvoidonFail(BletiaExceptionresult) { // Call when the request was failure. } });
    // BluetoothGattCharacteristic characteristic aracteristic(characteristic) .then(newDoneCallback<BluetoothGattCharacteristic>() { @OverridepublicvoidonDone(BluetoothGattCharacteristicresult) { // Call when the request was successfully. } }) .fail(newFailCallback<BletiaException>() { @OVerridepublicvoidonFail(BletiaExceptionresult) { // Call when the request was failure. } });
    // BluetoothGattCharacteristic haracteristic(characteristic) .then(newDoneCallback<BluetoothGattCharacteristic>() { @OverridepublicvoidonDone(BluetoothGattCharacteristic